Review



mycobacterium tuberculosis reference strain h37rv  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    ATCC mycobacterium tuberculosis reference strain h37rv
    Mycobacterium Tuberculosis Reference Strain H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pm41679834-42-1-6
    Average 98 stars, based on 2908 article reviews
    mycobacterium tuberculosis reference strain h37rv - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    other:

    Article Title: Probable role of naturally derived bergapten molecule promotes autophagy in Mycobacterium tuberculosis-infected macrophages via PI3K/Akt/mTOR pathway.
    Article Snippet: 1 Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamilnadu, India 2 Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai 600119, Tamilnadu, India Abstract According to the recent WHO figures, nearly 23% of the world’s population is infected with tuberculosis.. The causative pathogen Mycobacterium tuberculosis (Mtb) primarily resides in the macrophages of host cells and hinders the autophagy mechanism by preventing the fusion of phagolysosomes.. This study investigated the intracellular antituberculosis activity of bergapten (TPB1), isolated from Tridax procumbens, in human monocytic leukemia-derived (THP-1) cells, as well as its effects on the molecular expression of autophagy-related genes.

    Activity Assay:

    Article Title: Nifuroxazide and 4-Hydroxybenzhydrazone Derivatives as New Antiparasitic ( Trypanosoma cruzi and Leishmania mexicana ) and Anti- Mycobacterium tuberculosis Agents.
    Article Snippet: .. Anti-Mycobacterium Activity The reference strain H37Rv (ATCC 27294) of M. tuberculosis was utilized to assess anti-M. tuberculosis activity using the microplate Alamar Blue Assay (MABA), as previously reported [22,23]. ..

    Article Title: Nifuroxazide and 4-Hydroxybenzhydrazone Derivatives as New Antiparasitic ( Trypanosoma cruzi and Leishmania mexicana ) and Anti- Mycobacterium tuberculosis Agents
    Article Snippet: .. The reference strain H37Rv (ATCC 27294) of M. tuberculosis was utilized to assess anti- M. tuberculosis activity using the microplate Alamar Blue Assay (MABA), as previously reported [ , ]. ..

    Alamar Blue Assay:

    Article Title: Nifuroxazide and 4-Hydroxybenzhydrazone Derivatives as New Antiparasitic ( Trypanosoma cruzi and Leishmania mexicana ) and Anti- Mycobacterium tuberculosis Agents.
    Article Snippet: .. Anti-Mycobacterium Activity The reference strain H37Rv (ATCC 27294) of M. tuberculosis was utilized to assess anti-M. tuberculosis activity using the microplate Alamar Blue Assay (MABA), as previously reported [22,23]. ..

    Article Title: Nifuroxazide and 4-Hydroxybenzhydrazone Derivatives as New Antiparasitic ( Trypanosoma cruzi and Leishmania mexicana ) and Anti- Mycobacterium tuberculosis Agents
    Article Snippet: .. The reference strain H37Rv (ATCC 27294) of M. tuberculosis was utilized to assess anti- M. tuberculosis activity using the microplate Alamar Blue Assay (MABA), as previously reported [ , ]. ..

    Control:

    Article Title: QMAC-DST for Rapid Detection of Drug Resistance in Pulmonary Tuberculosis Patients: A Multicenter Pre–Post Comparative Study
    Article Snippet: .. The process included the reference strain H37Rv (ATCC 27294) for quality control. ..

    Cell Culture:

    Article Title: Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management
    Article Snippet: .. Mtb strains, including sub-lineages 4.1.2.1, 4.2.2.2, 4.6.3, lineages 3 and 7, and the reference strain H37Rv (ATCC 27294), were cultured in liquid 7H9 Middlebrook broth. ..

    Suspension:

    Article Title: Genomic Insight into Primary Adaptation of Mycobacterium tuberculosis to Aroylhydrazones and Nitrofuroylamides In Vitro
    Article Snippet: .. A suspension of the reference strain H37Rv (ATCC 25618, Manassas, VA, USA) was prepared at 10 8 CFU/mL, and 1 mL was applied to a solid Lowenstein–Jensen culture medium on a Petri dish with an increased concentration of a compound (two-fold to ten-fold MIC previously determined, see ); a detailed laboratory protocol is available on request from corresponding authors. ..

    Concentration Assay:

    Article Title: Genomic Insight into Primary Adaptation of Mycobacterium tuberculosis to Aroylhydrazones and Nitrofuroylamides In Vitro
    Article Snippet: .. A suspension of the reference strain H37Rv (ATCC 25618, Manassas, VA, USA) was prepared at 10 8 CFU/mL, and 1 mL was applied to a solid Lowenstein–Jensen culture medium on a Petri dish with an increased concentration of a compound (two-fold to ten-fold MIC previously determined, see ); a detailed laboratory protocol is available on request from corresponding authors. ..



    Similar Products

    mycobacterium tuberculosis reference strain h37rv  (ATCC)
    98
    ATCC mycobacterium tuberculosis reference strain h37rv
    Mycobacterium Tuberculosis Reference Strain H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pm41679834-42-1-6
    Average 98 stars, based on 1 article reviews
    mycobacterium tuberculosis reference strain h37rv - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    h37rv reference strain  (ATCC)
    99
    ATCC h37rv reference strain
    H37rv Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis+subsp%2E+tuberculosis/pmc12868246-102-11-14
    Average 99 stars, based on 1 article reviews
    h37rv reference strain - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    mtb h37rv reference strain  (ATCC)
    98
    ATCC mtb h37rv reference strain
    Schematic of the experimental workflow for method validation using <t>H37Rv-spiked</t> dust samples
    Mtb H37rv Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pmc12870882-49-1-10
    Average 98 stars, based on 1 article reviews
    mtb h37rv reference strain - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    drug susceptible reference strain h37rv  (ATCC)
    99
    ATCC drug susceptible reference strain h37rv
    Images of different contents of Ag@SBA-15/PDA and EPL cultured with M. tuberculosis <t>(H37Rv).</t>
    Drug Susceptible Reference Strain H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis+subsp%2E+tuberculosis/pmc12643096-81-1-5
    Average 99 stars, based on 1 article reviews
    drug susceptible reference strain h37rv - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    lineage 4 h37rv atcc 27294 reference strain  (ATCC)
    98
    ATCC lineage 4 h37rv atcc 27294 reference strain
    Images of different contents of Ag@SBA-15/PDA and EPL cultured with M. tuberculosis <t>(H37Rv).</t>
    Lineage 4 H37rv Atcc 27294 Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pmc12587575-22-7-10
    Average 98 stars, based on 1 article reviews
    lineage 4 h37rv atcc 27294 reference strain - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    mtb reference strain h37rv  (ATCC)
    99
    ATCC mtb reference strain h37rv
    The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of <t>H37Rv</t> after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.
    Mtb Reference Strain H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis+subsp%2E+tuberculosis/pmc12584698-47-0-4
    Average 99 stars, based on 1 article reviews
    mtb reference strain h37rv - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    m tuberculosis reference strain h37rv  (ATCC)
    99
    ATCC m tuberculosis reference strain h37rv
    The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of <t>H37Rv</t> after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.
    M Tuberculosis Reference Strain H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis+subsp%2E+tuberculosis/pmc12607706-89-12-17
    Average 99 stars, based on 1 article reviews
    m tuberculosis reference strain h37rv - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    reference strain m tuberculosis h37rv atcc 27294  (ATCC)
    99
    ATCC reference strain m tuberculosis h37rv atcc 27294
    The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of <t>H37Rv</t> after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.
    Reference Strain M Tuberculosis H37rv Atcc 27294, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+strain+h37rv/Mycobacterium+tuberculosis+subsp%2E+tuberculosis/pmc12584017-71-1-6
    Average 99 stars, based on 1 article reviews
    reference strain m tuberculosis h37rv atcc 27294 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Biomarker Discovery

    Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Centrifugation, Staining, Suspension, Comparison

    Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Incubation, Comparison

    Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Amplification, DNA Extraction, Extraction, MANN-WHITNEY

    Images of different contents of Ag@SBA-15/PDA and EPL cultured with M. tuberculosis (H37Rv).

    Journal: RSC Advances

    Article Title: Triple-synergy bactericidal action: a mannose-decorated bioinspired coating on silver-mesoporous silica for eradicating multidrug-resistant Mycobacterium tuberculosis

    doi: 10.1039/d5ra07047c

    Figure Lengend Snippet: Images of different contents of Ag@SBA-15/PDA and EPL cultured with M. tuberculosis (H37Rv).

    Article Snippet: The drug-susceptible reference strain H37Rv (ATCC 27294) was also used in this study.

    Techniques: Cell Culture

    The MIC results of concentrations of 4–512 µg mL −1 of Ag@SBA-15/PDA, EPL, Ag@SBA-15/PDA-EPL and Ag@SBA-15/Man-PDA-EPL against (A) H37Rv and (B) MDR strains of M. tuberculosis .

    Journal: RSC Advances

    Article Title: Triple-synergy bactericidal action: a mannose-decorated bioinspired coating on silver-mesoporous silica for eradicating multidrug-resistant Mycobacterium tuberculosis

    doi: 10.1039/d5ra07047c

    Figure Lengend Snippet: The MIC results of concentrations of 4–512 µg mL −1 of Ag@SBA-15/PDA, EPL, Ag@SBA-15/PDA-EPL and Ag@SBA-15/Man-PDA-EPL against (A) H37Rv and (B) MDR strains of M. tuberculosis .

    Article Snippet: The drug-susceptible reference strain H37Rv (ATCC 27294) was also used in this study.

    Techniques:

    The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of H37Rv after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.

    Journal: Microbiology Spectrum

    Article Title: Lefamulin harbors promising anti-tuberculosis activity against multidrug-resistant Mycobacterium tuberculosis isolates

    doi: 10.1128/spectrum.02250-25

    Figure Lengend Snippet: The bacteriostatic activity of LEF against M. tuberculosis . The MBC of LEF against M. tuberculosis: ( A ) the PI and DAPI fluorescence values ( B ) and bacterial mortality rate ( C ) of H37Rv after co-incubation with 1/8 × MIC, 1/4 × MIC, 1 × MIC, 4 × MIC, 8 × MIC, and 64 × MIC of LEF for 7 days are shown. All data are presented as the means ± SD ( n = 3). **** P < 0.0001.

    Article Snippet: Mtb reference strain H37Rv (ATCC 27294) and clinical isolated strains, including sensitive strains and MDR strains of Mtb, were stored in the Bio-bank in Beijing Chest Hospital.

    Techniques: Activity Assay, Fluorescence, Incubation

    Time-kill curves of LEF ( A ), BDQ ( B ), and INH ( C ) against M. tuberculosis H37Rv. Antibiotic concentrations are presented as different symbols. The DMSO was used as a NC.

    Journal: Microbiology Spectrum

    Article Title: Lefamulin harbors promising anti-tuberculosis activity against multidrug-resistant Mycobacterium tuberculosis isolates

    doi: 10.1128/spectrum.02250-25

    Figure Lengend Snippet: Time-kill curves of LEF ( A ), BDQ ( B ), and INH ( C ) against M. tuberculosis H37Rv. Antibiotic concentrations are presented as different symbols. The DMSO was used as a NC.

    Article Snippet: Mtb reference strain H37Rv (ATCC 27294) and clinical isolated strains, including sensitive strains and MDR strains of Mtb, were stored in the Bio-bank in Beijing Chest Hospital.

    Techniques: